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0:00 [Music] 0:02 thank you matilda 0:03 and thank you dr graviol 0:07 professor jackson do you like to talk 0:09 something 0:10 at this point i have nothing to add 0:14 except if there was some question that i 0:15 can answer otherwise 0:17 i thank you for organizing this session 0:23 thank you question 0:27 please is uh writing english 0:32 can you read the the commentary first 0:34 one 0:37 can you read the comments yeah but i 0:39 think that most of them are 0:40 in portuguese 0:44 no i asked you to write in english i 0:46 have 0:47 in the comments oh okay i was looking at 0:50 private chat 0:58 i'm going up the list oh boy lots of 1:00 comment 1:04 are you in comments yet yeah i'm just 1:06 looking at the comments very quickly 1:09 uh grow up 1:12 anachrestia is is is the probability 1:16 that the prospered test will give false 1:19 negative results that's a 1:21 question mostly for gabriella gabriel 1:24 can you answer that 1:27 uh so at the moment we don't entirely 1:30 know with my test 1:32 generally um 1:35 tests similar to this one have a 1:37 sensitivity of about 1:39 90 so the probability of giving a 1:42 negative result is very low 1:44 because it also tends to have a 1:45 sensibility of about 1:47 80 to 90 as well so overall it's better 1:51 than some of the alternatives that exist 1:52 that aren't rtq bcr okay another 1:55 question for you 1:57 it's also from anna christina does the 2:00 crisper test 2:01 apply to other disease like ebola 2:05 zika or dank 2:08 so we actually intend to adapt the test 2:11 to ebola 2:12 as soon as we can um we're toying with 2:15 the idea of doing zika 2:16 leps and a few others because all we 2:19 need to do is change a few of the 2:20 sequences that we're using 2:22 so the lamp primers and the guide for 2:24 the 2:25 crisper cast 13 protein and at that 2:28 point we'll immediately be able to start 2:30 detecting those viruses as well 2:32 so now the test exists it's just a 2:34 matter of optimizing it for every 2:35 individual virus okay there's another 2:39 question from france 2:40 antarctic nouvelle what are the 2:43 perspective of using the crispr castline 2:46 technology 2:47 to treat hiv i must admit i'm not 2:51 an expert of hiv i know that some groups 2:54 are working on that 2:56 i don't know what are the chance that 2:58 whether or not 2:59 this will work but i i think this is 3:02 very zonable you know the bacteria 3:04 have been using the crispr cation 3:06 technology since millions of years 3:08 to cut the virus and so the idea here is 3:12 to use the cascading technology 3:14 to cross to cut the hiv virus 3:17 i think this is quite relatable in fact 3:19 at the beginning of the cobia 19 3:21 pandemic i saw a group in the united 3:25 states 3:25 that were proposing to to use the crispr 3:29 cast 9 technology 3:31 indeed to cut the copy 19 virus so 3:34 it's the same approach for the hiv i 3:37 think this is a 3:38 reasonable approach my lab is not 3:40 working on that approach 3:46 also from convention uh can this 3:49 technology substitute to be antibiotic 3:52 in the near future for example to treat 3:55 multi-resistant tuberculosis 3:59 again i think it's a good suggestion and 4:01 yes indeed 4:03 the main problem with the with these 4:05 crispr cas9 technology 4:08 is to find an adequate technique to 4:10 deliver 4:11 either the cast 9 protein the cast iron 4:13 gene 4:14 and so far in gene therapy what is being 4:18 used is the adeno associated virus 4:21 unfortunately i didn't know associated 4:23 virus custard fortune to produce 4:26 in one manufacturing practice so we have 4:29 to find another 4:30 delivery method to deliver into the cas9 4:33 protein or the cas9 gene 4:35 and then it would ease using it because 4:38 right now it would be 4:39 just too expensive to be used instead of 4:42 an 4:42 antibiotic 4:46 also from fred synthetic 4:49 crispr cast 9 may be an option in the 4:52 near future 4:53 to treat kobe 4:58 well all of that i think is feasible 5:01 if i would have more money in my lab we 5:04 could start working on that 5:05 also i i think these are a reasonable 5:08 project 5:10 many of the comments are in portuguese 5:12 unfortunately 5:17 there is no question for this only 5:20 comments 5:21 let me 5:34 yeah i see that i vary between two and 5:38 five stars 5:43 well one question from vitor is it uh 5:47 like to know it is i'd like to know if 5:50 crispr 5:51 is affordable to public health system 5:54 and if it can contribute to the cost 5:56 reducing of genetic therapy 5:59 that already exists 6:02 the main cause of gene therapy that 6:05 currently exists 6:07 is the production of the adeno 6:09 associated virus 6:10 in good manufacturing practice condition 6:13 the last time i look into doing a 6:16 clinical trial 6:17 for a systemic delivery of some gene 6:20 therapy 6:22 it was about a million dollar to produce 6:26 enough aav to treat one patient so to do 6:29 a clinical trial on 10 patients 6:31 you needed to just to start a budget of 6:34 10 million 6:36 and of course so far i have not been 6:38 able to obtain 6:39 such large budget the only 6:42 people that have such large budget are 6:45 american companies 6:47 that will spend a fortune doing these 6:50 clinical trials and they have millions 6:51 of dollars 6:52 available the problem is indeed that 6:55 after 6:56 the treatment has been demonstrated 6:58 effective 7:00 the cost of the treatment is 23 million 7:02 dollars per person 7:04 so i i think it's it's 7:07 the future we need to develop gene 7:09 therapy for 7:10 many many idiots every disease but we 7:13 also need to develop 7:14 a cheap gene therapy you know i i i 7:17 think ideally 7:18 a gene therapy should be around 100 000 7:22 not a million dollar because most of the 7:25 health public health system 7:27 cannot afford to do that i think that 7:29 it's only in in united states 7:31 where rich people can afford to pay for 7:34 those 7:35 and poor people will not get access to 7:37 the treatment 7:40 [Music] 7:42 still looking down for the list to see 7:44 if there is any other question 7:49 excuse me professor jax uh 7:52 just to make a small comment here for 7:54 those who doesn't speak 7:55 english i'll try to get the general idea 7:59 from 8:00 the answers you just gave and grab your 8:02 gabriel also 8:03 uh just a second 8:10 principalization 8:18 [Music] 8:24 foreign 9:20 um 9:49 thank you for your time and if is there 9:52 any question you can 9:53 keep uh reading and answering them 9:56 well if there are other questions people 10:00 can always send me some 10:02 emails okay 10:05 uh thank you professor jack england 10:08 for this wonderful meeting thank you 10:11 gabrielle 10:12 thank you barbara dr barbara oliver 10:17 thank you matthieu shots and i hope you 10:20 have another 10:22 another one meeting like this 10:25 thank you thank you very much thank you 10:29 goodbye thank you 10:44 is 11:15 died